{"id":30949,"date":"2023-03-06T14:58:42","date_gmt":"2023-03-06T07:58:42","guid":{"rendered":"https:\/\/www.oucru.org\/project\/40tb-su-dung-ky-thuat-giai-trinh-tu-dich-moi-flash-mtb-de-phat-hien-som-vi-khuan-lao-khang-thuoc-trong-dom\/"},"modified":"2023-07-04T14:38:34","modified_gmt":"2023-07-04T07:38:34","slug":"40tb-su-dung-ky-thuat-giai-trinh-tu-dich-moi-flash-mtb-de-phat-hien-som-vi-khuan-lao-khang-thuoc-trong-dom","status":"publish","type":"project","link":"https:\/\/www.oucru.org\/vi\/project\/40tb-su-dung-ky-thuat-giai-trinh-tu-dich-moi-flash-mtb-de-phat-hien-som-vi-khuan-lao-khang-thuoc-trong-dom\/","title":{"rendered":"(40TB) S\u1eed d\u1ee5ng k\u1ef9 thu\u1eadt gi\u1ea3i tr\u00ecnh t\u1ef1 \u0111\u00edch m\u1edbi FLASH-Mtb \u0111\u1ec3 ph\u00e1t hi\u1ec7n s\u1edbm vi khu\u1ea9n lao kh\u00e1ng thu\u1ed1c trong \u0111\u1eddm"},"content":{"rendered":"<h1>B\u1ed1i c\u1ea3nh nghi\u00ean c\u1ee9u<\/h1>\n<p>Gi\u1ea3i tr\u00ecnh t\u1ef1 to\u00e0n b\u1ed9 b\u1ed9 gen (WGS) d\u1ef1a tr\u00ean \u0111\u1eddm tr\u1ef1c ti\u1ebfp c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n lao kh\u00e1ng thu\u1ed1c trong 30-40% m\u1eabu. Tuy nhi\u00ean, so v\u1edbi gi\u1ea3i tr\u00ecnh t\u1ef1 to\u00e0n b\u1ed9 b\u1ed9 gen d\u1ef1a tr\u00ean nu\u00f4i c\u1ea5y ho\u1eb7c kh\u00e1ng sinh \u0111\u1ed3 (DST), hi\u1ec7u su\u1ea5t c\u1ee7a WGS d\u1ef1a tr\u00ean \u0111\u1eddm ch\u01b0a \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 \u0111\u00fang.<\/p>\n<p>FLASH-Mtb c\u00f3 th\u1ec3 gi\u00fap c\u1ea3i thi\u1ec7n \u0111\u1ed9 ch\u00ednh x\u00e1c c\u1ee7a vi\u1ec7c g\u1ecdi bi\u1ebfn th\u1ec3 li\u00ean quan \u0111\u1ebfn kh\u00e1ng thu\u1ed1c trong c\u00e1c m\u1eabu, ngay c\u1ea3 trong c\u00e1c m\u1eabu c\u00f3 t\u1ea3i l\u01b0\u1ee3ng Mtb r\u1ea5t th\u1ea5p. Ngo\u00e0i ra, ph\u01b0\u01a1ng ph\u00e1p FLASH linh ho\u1ea1t \u0111\u1ec3 s\u1eeda \u0111\u1ed5i v\u1ec1 lo\u1ea1i v\u00e0 s\u1ed1 l\u01b0\u1ee3ng m\u1ee5c ti\u00eau quan t\u00e2m. Do \u0111\u00f3, s\u1ef1 th\u00e0nh c\u00f4ng c\u1ee7a nghi\u00ean c\u1ee9u th\u00ed \u0111i\u1ec3m n\u00e0y c\u00f3 th\u1ec3 gi\u00fap ph\u01b0\u01a1ng ph\u00e1p n\u00e0y s\u1eb5n s\u00e0ng \u0111\u1ec3 th\u1eed nghi\u1ec7m t\u1ea1i c\u00e1c b\u1ec7nh vi\u1ec7n h\u1ee3p t\u00e1c v\u1edbi ch\u00fang t\u00f4i \u1edf H\u00e0 N\u1ed9i v\u00e0 TP.HCM.<\/p>\n<h1>M\u1ee5c ti\u00eau<\/h1>\n<ul>\n<li>\u0110\u1ec3 ch\u1ee9ng minh r\u1eb1ng trong c\u00e1c m\u1eabu \u0111\u1eddm tr\u1ef1c ti\u1ebfp t\u1eeb b\u1ec7nh nh\u00e2n lao, FLASH-Mtb tr\u00ecnh t\u1ef1 \u0111\u00edch c\u00f3 th\u1ec3 v\u01b0\u1ee3t tr\u1ed9i so v\u1edbi gi\u1ea3i tr\u00ecnh t\u1ef1 to\u00e0n b\u1ed9 b\u1ed9 gen v\u1ec1 t\u1ef7 l\u1ec7 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ed9 ch\u00ednh x\u00e1c trong ch\u1ea9n \u0111o\u00e1n t\u00ednh nh\u1ea1y c\u1ea3m v\u1edbi thu\u1ed1c.<\/li>\n<li>\u0110\u1ec3 ch\u1ee9ng minh r\u1eb1ng FLASH-Mtb c\u1ee7a \u0111\u1eddm c\u00f3 th\u1ec3 d\u1ef1 \u0111o\u00e1n \u0111\u1ed9 nh\u1ea1y v\u1edbi thu\u1ed1c ch\u00ednh x\u00e1c nh\u01b0 gi\u1ea3i tr\u00ecnh t\u1ef1 to\u00e0n b\u1ed9 b\u1ed9 gen v\u00e0 kh\u00e1ng sinh \u0111\u1ed3 c\u1ee7a m\u1eabu c\u1ea5y c\u1ee7a ch\u00fang nh\u01b0ng \u1edf th\u1eddi \u0111i\u1ec3m s\u1edbm h\u01a1n nhi\u1ec1u.<\/li>\n<li>\u0110\u1ec3 ch\u1ee9ng minh r\u1eb1ng trong \u0111\u1eddm, FLASH-Mtb c\u00f3 th\u1ec3 v\u01b0\u1ee3t tr\u1ed9i so v\u1edbi x\u00e9t nghi\u1ec7m Deeplex th\u01b0\u01a1ng m\u1ea1i v\u1ec1 t\u1ef7 l\u1ec7 ph\u00e1t hi\u1ec7n v\u00e0 chi ph\u00ed ch\u1ea9n \u0111o\u00e1n \u0111\u1ed9 nh\u1ea1y v\u1edbi thu\u1ed1c.<\/li>\n<\/ul>\n<h1>Ph\u01b0\u01a1ng ph\u00e1p<\/h1>\n<p>C\u00e1c m\u1eabu \u0111\u1eddm tr\u01b0\u1edbc khi \u0111i\u1ec1u tr\u1ecb c\u1ee7a b\u1ec7nh nh\u00e2n lao ph\u1ed5i s\u1ebd \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng. T\u1ea3i l\u01b0\u1ee3ng vi khu\u1ea9n lao s\u1ebd \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh b\u1eb1ng k\u00ednh hi\u1ec3n vi v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng b\u1eb1ng x\u00e9t nghi\u1ec7m t\u1ea3i l\u01b0\u1ee3ng vi khu\u1ea9n ph\u00e2n t\u1eed rRNA 16s (MBLA). DNA t\u1eeb \u00edt nh\u1ea5t 1 ml \u0111\u1eddm s\u1ebd \u0111\u01b0\u1ee3c chi\u1ebft xu\u1ea5t v\u00e0 gi\u1ea3i tr\u00ecnh t\u1ef1 b\u1eb1ng gi\u1ea3i tr\u00ecnh t\u1ef1 to\u00e0n b\u1ed9 b\u1ed9 gen v\u00e0 hai ph\u01b0\u01a1ng ph\u00e1p gi\u1ea3i tr\u00ecnh t\u1ef1 nh\u1eafm \u0111\u00edch l\u00e0 Deeplex v\u00e0 FLASH-Mtb. Vi khu\u1ea9n lao t\u1eeb \u0111\u1eddm c\u0169ng s\u1ebd \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y v\u00e0 c\u00e1c m\u1eabu d\u01b0\u01a1ng t\u00ednh s\u1ebd \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 th\u1ef1c hi\u1ec7n kh\u00e1ng sinh \u0111\u1ed3 ki\u1ec3u h\u00ecnh v\u00e0 gi\u1ea3i tr\u00ecnh t\u1ef1 to\u00e0n b\u1ed9 b\u1ed9 gen nu\u00f4i c\u1ea5y DNA.<\/p>\n","protected":false},"author":28,"featured_media":0,"menu_order":0,"template":"","meta":{"_acf_changed":false,"_links_to":"","_links_to_target":""},"tags":[579,608],"project_category":[341],"class_list":["post-30949","project","type-project","status-publish","hentry","tag-hoan-thanh","tag-nghien-cuu-trong-phong-xet-nghiem","project_category-lao-phoi"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.3.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>(40TB) S\u1eed d\u1ee5ng k\u1ef9 thu\u1eadt gi\u1ea3i tr\u00ecnh t\u1ef1 \u0111\u00edch m\u1edbi FLASH-Mtb \u0111\u1ec3 ph\u00e1t hi\u1ec7n s\u1edbm vi khu\u1ea9n lao kh\u00e1ng thu\u1ed1c trong \u0111\u1eddm - OUCRU<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.oucru.org\/vi\/project\/40tb-su-dung-ky-thuat-giai-trinh-tu-dich-moi-flash-mtb-de-phat-hien-som-vi-khuan-lao-khang-thuoc-trong-dom\/\" \/>\n<meta property=\"og:locale\" content=\"vi_VN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"(40TB) S\u1eed d\u1ee5ng k\u1ef9 thu\u1eadt gi\u1ea3i tr\u00ecnh t\u1ef1 \u0111\u00edch m\u1edbi FLASH-Mtb \u0111\u1ec3 ph\u00e1t hi\u1ec7n s\u1edbm vi khu\u1ea9n lao kh\u00e1ng thu\u1ed1c trong \u0111\u1eddm - OUCRU\" \/>\n<meta property=\"og:description\" content=\"B\u1ed1i c\u1ea3nh nghi\u00ean c\u1ee9u Gi\u1ea3i tr\u00ecnh t\u1ef1 to\u00e0n b\u1ed9 b\u1ed9 gen (WGS) d\u1ef1a tr\u00ean \u0111\u1eddm tr\u1ef1c ti\u1ebfp c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n lao kh\u00e1ng thu\u1ed1c trong 30-40% m\u1eabu. 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