Tuberculosis (TB) remains a leading cause of death in low-and-middle-income countries, including Nepal. Although new TB vaccines are in clinical development, little is known about public willingness to accept them. An exploratory qualitative study was conducted to explore the enablers and barriers for public acceptance of a hypothetical new TB vaccine among the community of three TB-affected districts in Nepal in collaboration with Birat Nepal Medical Trust (BNMT). A total of six focus group discussions (FGD) with 60 heterogenous population group was conducted. While OUCRU Nepal led the study, data collection was conducted by BNMT Nepal. The study has been completed, and the manuscript of the study is under review at BMJ Public Health.